CEACAM-5/CD66e, Recombinant, Human (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5)
Biozol Catalog Number:
USB-052073
Supplier Catalog Number:
052073
Alternative Catalog Number:
USB-052073-50
Manufacturer:
US Biological
Category:
Molekularbiologie
CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species. Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor. CEACAM-5 is synthesized as a 180kD, variably glycosylated molecule of which approximately 60% is carbohydrate. CEACAM-5 functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors. This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors and is a commonly used cancer marker. It promotes tumor cell migration, invasion, adhesion, and metastasis. It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis. Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis. CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins. These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact. Recombinant protein corresponding to Lys35-Ala685 with an C-Terminal 6X His-tag from human CEACAM-5/CD66e, expressed in NS0 cells. Uniprot/Accession: Q8N4D0. Activity: Measured by its binding activity in a functional ELISA. Ideo, H. et al. (2005) J. Biol. Chem. 280:4730. Recombinant Human CEACAM-5/CD66e can bind Recombinant Human Galectin-4 with an estimated Kd <4 nM. Molecular Weight: ~155-180kD, reducing conditions Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
