It is thought that the complement system might play a role in many diseases with an immune component, such as Alzheimers disease, asthma, lupus erythematosus, various forms of arthritis, autoimmune heart disease and multiple sclerosis. Complement C4 (200kD) is composed of three polypeptide chains - alpha (93kD), beta (78kD), and gamma (33kD). During complement activation, C4 is cleaved by the serine protease C1s to C4a (9kD) and C4b (190kD). C4b is further cleaved by factor I to C4d (45kD) and C4c (140kD). The current protocol yields C4c, however intact C4 and/or C4b and C4d may be co-purified. Protein: 1-5mg/mL (E0.1%280nm = 0.92) Identity: Confirmed by Western Blot Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
147
Purity:
95% (SDS-PAGE)
Form:
Supplied as a liquid in PBS, pH 7.4.
* VAT and and shipping costs not included. Errors and price changes excepted
