Proteome Profiler Ubiquitin Array BioAssay(TM) (Human)

Product Overview

• Article Name: Proteome Profiler Ubiquitin Array BioAssay(TM) (Human)
• Biozol Catalog Number: USB-052381
• Supplier Catalog Number: 052381
• Alternative Catalog Number: USB-052381-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Analyzing the expression profile of ubiquitinated proteins is essential for understanding their roles in normal cellular responses and the development of disease states. The Proteome Profiler Ubiquitin Array BioAssay(TM) (Human) is a rapid, sensitive, and economical tool to simultaneously detect changes in ubiquitinated proteins between samples. The relative ubiquitination levels of 49 different proteins can be determined without performing numerous immunoprecipitations and Western blots. Each capture antibody was carefully selected using lysate samples prepared from cell lines known to express the target proteins. Principle of the Assay: Capture and control antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are diluted and incubated overnight with the Human Cell Ubiquitin Array. The membrane is washed to remove unbound proteins, followed by incubation with a biotinylated pan anti-ubiquitin detection antibody. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the relative amount of ubiquitinated protein bound. Refer to the Appendix for a list and coordinates of analytes and controls. Kit Components: Human Ubiquitin Array: 4 nitrocellulose membranes each containing 49 different capture antibodies printed in duplicate. Array Buffer 1: 21ml of a buffered protein base with preservatives. Array Buffer 2 5X Concentrate: 21ml of a concentrated buffered protein base with preservatives. Lysis Buffer 6: 21ml of a denaturing buffered solution. Wash Buffer Concentrate: 2 vials (21ml/vial) of a 25-fold concentrated solution of buffered surfactant with preservative. May turn yellow over time. Detection Antibody, Human Ubiquitin Array: 1 vial of biotinylated detection antibody, lyophilized. Streptavidin-HRP: 200ul Chemi Reagent 1: 1 vial (2.5 mL) Chemi Reagent 2: 1 vial (2.5 mL) 4-Well Multi-dish Clear 4-well rectangular multi-dish. Transparency Overlay Template Storage and Stability: Unused kit is stable for 3 months at 4C. Once opened may be stored for up to 3 months at 4C, return unused membranes to the foil pouch containing the dessicant pack and reseal along entire edge of the zip seal.