Albumin (BSA, ALB) (Low Endotoxin and Protease-Free)

Product Overview

• Article Name: Albumin (BSA, ALB) (Low Endotoxin and Protease-Free)
• Biozol Catalog Number: USB-052414
• Supplier Catalog Number: 052414
• Alternative Catalog Number: USB-052414-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Bovine serum albumin can be used as a diluent or a blocking agent in numerous applications including ELISA, blots and immunohistochemistry. In restriction digests, bovine serum albumin is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. BSA doesnt affect other enzymes that do not need it for stabilization and is used because of its stability. Albumins are readily soluble in de-ionized water and can only precipitated by high concentrations of neutral salts. Bovine Serum Albumin help stabilize other solubilized proteins. Bovine Serum Albumin (BSA) is readily coagulated with heat. BSA as with other albumins are acidic proteins which occur in abundance in body fluids and tissues and is easily crystallized. Source: Bovine Plasma Protein: 98% (Elemental analysis, dry basis) Analysis: Sodium: Reported mg/g Potassium: Reported mg/g Chloride: Reported mg/g Calcium: Reported mg/g pH: 6.5-7.5 (10% solution) Water: 5% (Loss on drying) Protease: 0.005U/mg (Enzymatic) Bovine IgG: Not detected (Turbidimetric) Ash: <2% (Residue on ignition) Endotoxin: 3EU/mg (LAL) Heavy Metals: 10 ppm as Pb (ICP) Purity: 98% (Electrophoresis) Solubility: Clear to slightly hazy, yellow to yellow-green (4% solution in water) Appearance: White to yellow, tan to green cast powder Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.