Recombinant protein corresponding to aa17-242 from human Cluster of Differentiation 42d, fused to His-Tag at N-terminal, expressed in E. coli. Uniprot/Accession: P40197 Predicted Molecular Weight: 25.6kD Accurate Molecular Weight: 27kD as determined by SDS-PAGE under reducing conditions Subcellular Localization: Membrane Predicted Isoelectric Point: 9.5 Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS, pH 7.4 to a concentration of 0.1-1mg/ml. Do not vortex.
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