Bone Morphogenetic Protein 6, Recombinant, Human, Active (BMP-6, VGR1, BMP6) (BSA Free)
Biozol Catalog Number:
USB-052543
Supplier Catalog Number:
052543
Alternative Catalog Number:
USB-052543-20
Manufacturer:
US Biological
Category:
Molekularbiologie
Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). Human BMP-6 is synthesized as a 513 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18kD C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, although it can also form heterodimers with mature BMP-2 (2, 3). Mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (4, 5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP coreceptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3). Recombinant protein corresponding to Gln382-His513 from human BMP-6, expressed in mouse myeloma cell line, NSO. Swiss/UniProt Accession: P22004 Predicted Molecular Mass: ~15kD SDS-PAGE: ~18kD, reducing conditions N-Terminal Sequence Analysis: Gln382 Biological Activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.02-0.15ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
