Neurofilament Light, Recombinant, Human, His-Tag (NF-L, Nfl, NEFL)
Catalog Number:
USB-052723
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| Article Name: | Neurofilament Light, Recombinant, Human, His-Tag (NF-L, Nfl, NEFL) |
| Biozol Catalog Number: | USB-052723 |
| Supplier Catalog Number: | 052723 |
| Alternative Catalog Number: | USB-052723-50 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Application: | ELISA |
| Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of four major proteins called NF-L, NF-M, NF-H and alpha-internexin. NF-L, NF-M and NF-H were named based on their apparent molecular weight on SDS-PAGE gels, so NF-L is low or light, NF-M is medium or middle and NF-H is high or heavy. On SDS-PAGE NF-L runs at 68-70kD, NF-M at 145-160kD and NF-H at 200-220kD with some species variability, larger species tending to have larger molecules. In every case the real molecular weight is significantly lower since long acidic sequences in these molecules cause them to run aberrantly. These three proteins are major components of large diameter axons in the adult, while alpha-internexin is a more major component of the developing nervous system, although still present in the adult. NF-L and other neurofilament subunits accumulate in many neurological diseases, such as Lou Gehrigs disease (ALS) and Alzheimers disease, and mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2-4). NF-L is a very abundant protein particularly concentrated in large diameter axons and may leak into blood and CSF following various kinds of axonal injury and/or degeneration. There has therefore been much recent interest in the detection of NF-L in CSF and blood as a surrogate marker of neuronal damage and degeneration (5). NF-L is also known as NF-Light, Nfl and NEFL. Recombinant protein corresponding to human NEFL, fused to His-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: P07196 Molecular Weight: ~70kD by SDS-PAGE plus ~5kD His Tag. Amino Acid Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFMSSFSYEP YYSTSYKRRY VETPRVHISS VRSGYSTARS AYSSYSAPVS SLSVRRSYS SSSGSLMPSL ENLDLSQVAA ISNDLKSIRT QEKAQLQDLN DRFASFIERV HELEQQNKVL EAELLVLRQK HSEPSRFRAL YEQEIRDLRL AAEDATNEKQ ALQGEREGLE ETLRNLQARY EEEVLSREDA EGRLMEARKG ADEAALARAE LEKRIDSLMD EISFLKKVHE EEIAELQAQI QYAQISVEMD VTKPDLSAAL KDIRAQYEKL AAKNMQNAEE WFKSRFTVLT ESAAKNTDAV RAAKDEVSES RRLLKAKTLE IEACRGMNEA LEKQLQELED KQNADISAMQ DTINKLENEL RTTKSEMARY LKEYQDLLNV KMALDIEIAA YRKLLEGEET RLSFTSVGSI TSGYSQSSQV FGRSAYGGLQ TSSYLMSTRS FPSYYTSHVQ EEQIEVEETI EAAKAEEAKD EPPSEGEAEE EEKDKEEAEE EEAAEEEEAA KEESEEAKEE EEGGEGEEGE ETKEAEEEEK KVEGAGEEQA AKKKD Theoretical pI: 4.69 Applications: Suitable for use in MSD, as a protein standard for ELISA and as an Immunogen for antibody production. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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