Dengue Virus, Pan (1-4), Clone: [323], Mouse, Monoclonal
Biozol Catalog Number:
USB-052899
Supplier Catalog Number:
052899
Alternative Catalog Number:
USB-052899-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, FC
Immunogen:
Purified recombinant Dengue virus NS1 protein, expressed in mammalian cells.
Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48kD glycoprotein (aa776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
