For the quantitative determination of bovine Interleukin 10 in serum, plasma, and tissue homogenates. Sensitivity: 2.5pg/ml Detection Range: 5-1000pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of bovine IL-10. No significant cross-reactivity or interference between bovine IL-10 and analogues was observed. Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-10 and Horseradish Peroxidase (HRP) conjugated IL-10. The competitive inhibition reaction is launched between with HRP labeled IL-10 and unlabeled IL-10 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-10 in the sample. The color development is stopped and the intensity of the color is measured. Kit Components: Microtiter Strips, 1x96 wells Standard, 6x0.5ml Antibody, 1x6ml HRP-conjugate, 1x6ml Wash Buffer (20X), 1x15ml Substrate A, 1x7ml Substrate B, 1x7ml Storage and Stability: Store all kit components at 4C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
