Recombinant protein corresponding to aa453-560 with an N-Terminal His-Tag from human AMH expressed in E. coli.
Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSG SEF-AGATAAD GPCALRELSV DLRAERSVLI PETYQANNCQ GVCGWPQSDR NPRYGNHVVL LLKMQVRGAA LARPPCCVPT AYAGKLLISL SEERISAHHV PNMVATECGC R Positive Control (Supplied): 139283A: Suppled as a recombinant protein (aa453-560) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by affinity chromatography
Form:
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
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