A2M/alpha2-Macroglobulin BioAssay(TM) ELISA Kit, Human
Biozol Catalog Number:
USB-143702A
Supplier Catalog Number:
143702A
Alternative Catalog Number:
USB-143702A-96
Manufacturer:
US Biological
Category:
Kits/Assays
Sandwich ELISA quantitative detection kit for Human A2M/alpha2-Macroglobulin in cell culture supernates, serum and plasma (heparin, EDTA). Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Assay Range: 625pg/ml-40,000pg/ml Sensitivity: < 20 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P01023 Entrez Gene ID: 2 Kit Components: 96-well plate pre-coated with anti-A2M/alpha2-Macroglobulin, Human Lyophilized recombinant A2M/alpha2-Macroglobulin, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
