Sandwich ELISA quantitative detection kit for Mouse DKK1 in cell culture supernates, serum and plasma (heparin, EDTA). Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Assay Range: 46.9pg/ml-3000pg/ml Sensitivity: < 15 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: O54908 Entrez Gene ID: 13380 Kit Components: 96-well plate pre-coated with anti-DKK1, Mouse Lyophilized recombinant DKK1, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
