Heat Shock Protein 27 (HSP27) BioAssay(TM) ELISA Kit (Human)
Biozol Catalog Number:
USB-143862
Supplier Catalog Number:
143862
Alternative Catalog Number:
USB-143862-96
Manufacturer:
US Biological
Category:
Kits/Assays
Sandwich ELISA quantitative detection kit for Human HSP27 in cell lysates. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kD. Assay Range: 62.5pg/ml-4000pg/ml Sensitivity: < 5 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P04792 Entrez Gene ID: 3315 Kit Components: 96-well plate pre-coated with anti-HSP27, Human Lyophilized recombinant HSP27, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
