Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells. Intended Use: For quantitative detection of human IL-27 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Range: 156pg/ml-10,000pg/ml Sensitivity: <10pg/ml Specificity: No detectable crossreactivity with other cytokine. Kit Components: 143914A: Microtiter Strip 1x96wells pre-coated with anti-IL-27, Human 143914B: Standard, Recombinant IL-27 Human, 10ng, 2x1 vial Lyophilized 143914C: Anti-human IL-27 (Biotin), 1x130ul USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution) 143697: Sample diluent buffer, 1x30ml 143698: Antibody diluent buffer, 1x12ml 143699: ABC diluent buffer, 1x12ml 143700: TMB color developing agent, 1x10ml 143701: TMB stop solution, 2N H2SO4, 1x10ml Storage and Stability: Store *143747B powder at 4C. Once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add 100ul of samples and standards and incubate the plate at 37C for 90 min. Do not wash. 2. Add 100ul biotinylated antibody and incubate the plate at 37C for 60 min. Wash plate 3 times with 0.01M TBS or PBS. 3. Add ABC working solution and incubate the plate at 37C for 30min. Wash plate 5 times with 0.01M TBS or PBS. 4. Add TMB color developing agent and incubate the plate at 37C in dark for 20-25 min. 5. Add 100ul TMB stop solution and read.
