Sandwich ELISA quantitative detection kit for Mouse IL-7 in cell culture supernates, serum and plasma (EDTA). Interleukin 7 (IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17 (T(H)17) cells in experimental autoimmune encephalomyelitis (EAE).2 Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 1 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P10168 Entrez Gene ID: 16196 Kit Components: 96-well plate pre-coated with anti-IL-7, Mouse Lyophilized recombinant IL-7, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
