Sandwich ELISA quantitative detection kit for Mouse MCP-1 in cell culture supernates and serum. Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1.1 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.2 Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1.3 The standard used in this kit is recombinant mouse MCP-1(Q24-R96) with the molecular mass of 8.5kD. Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 6 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P10148 Entrez Gene ID: 20296 Kit Components: 96-well plate pre-coated with anti-MCP-1, Mouse Lyophilized recombinant MCP-1, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
