Sandwich ELISA quantitative detection kit for Mouse Neurotrophin-3 in cell culture supernates and serum. Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6. Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 2 pg/ml Crossreactivity: There is cross-reactivates with NT-4 <2.5%, and no detectable cross-reactivity with any other cytokine. Uniprot ID: P20181 Entrez Gene ID: 18205 Kit Components: 96-well plate pre-coated with anti-Neurotrophin-3, Mouse Lyophilized recombinant Neurotrophin-3, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
