Sandwich ELISA quantitative detection kit for Rat NGF/NGF beta in cell culture supernates and serum. Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2 Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 1 pg/ml Crossreactivity: There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4. Uniprot ID: P25427 Entrez Gene ID: 310738 Kit Components: 96-well plate pre-coated with anti-NGF/NGF beta, Rat Lyophilized recombinant NGF/NGF beta, Rat standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
