Sandwich ELISA quantitative detection kit for Human Resistin in cell culture supernates, serum and plasma (heparin, EDTA). Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53kD. The detected Resistin includes zymogen and active enzyme. Assay Range: 78pg/ml-5,000pg/ml Sensitivity: < 3 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: Q9HD89 Entrez Gene ID: 56729 Kit Components: 96-well plate pre-coated with anti-Resistin, Human Lyophilized recombinant Resistin, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
