Sandwich ELISA quantitative detection kit for Human TGF alpha in cell culture supernates, serum, plasma (heparin, EDTA) and human milk. Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGFalpha stimulates neural cell proliferation in the adult injured brain.1 Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma.2 Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.3 Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 1 pg/ml Crossreactivity: There is cross-reactivates with TGFbeta2, TGFbeta3, TGFbeta5 <1%. Uniprot ID: P01135 Entrez Gene ID: 7039 Kit Components: 96-well plate pre-coated with anti-TGF alpha, Human Lyophilized recombinant TGF alpha, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
