GDF-8, BioAssay(TM) ELISA Kit (Myostatin, MSTN, Growth/Differentiation Factors, GDF-1 to GDF-15)

Product Overview

• Article Name: GDF-8, BioAssay(TM) ELISA Kit (Myostatin, MSTN, Growth/Differentiation Factors, GDF-1 to GDF-15)
• Biozol Catalog Number: USB-145740
• Supplier Catalog Number: 145740
• Alternative Catalog Number: USB-145740-1
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems. Intended Use: For the quantitative determination of Growth Differentiation Factor 8 (GDF-8) concentrations in cell culture supernates, tissue homogenates, serum, and plasma. Sensitivity: 5.32pg/ml Range: ~31.3-2000pg/ml Test Principle: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mature GDF-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any GDF-8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for mature GDF-8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDF-8 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Microplate, 1x96wells Conjugate (HRP), 1x21ml Standard, 1x20ng Assay Diluent, 1x11ml Calibrator Diluent concentrate, 1x21ml Wash Buffer concentrate, 1x21ml Color Reagent A, 1x12ml Color Reagent B, 1x12ml Stop Solution, 1x6ml Plate Sealers, 1x4 adhesive strips Storage and Stability: Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.