| TLR10 (Tolllike receptor 10, designated CD290) is a 90100kD type I transmembrane glycoprotein that is a member of the TLR1 subfamily within the TLR family of pathogenassociated molecular pattern (PAMP) receptors (13). Human TLR10 is synthesized as a precursor with a 19 amino acid (aa) signal sequence, an extracellular domain (ECD, aa 20576) with 15 or more leucinerich repeats (LRR), an LRRCT (LRR Cterminal) domain and nine potential sites for Nlinked glycosylation, a transmembrane domain, and a cytoplasmic region (aa 598811) with a TIR (Toll/IL1 receptor) domain (1, 3). Human TLR10 shares 71%, 81%, 82% and 83% aa sequence identity with rat, bovine, porcine and canine TLR10, respectively. The mouse TLR10 gene is nonfunctional (2). TLR10 is expressed on B cells and cell lines, except for early preB cells, and is more highly expressed on activated than resting cells (2, 4). It is also expressed on FOXP3+ regulatory T cells, placental trophoblasts, and CD1a+ and plasmacytoid dendritic cells (DC), but not on monocytederived DC (2, 5, 6). Among PAMPs, TLR10 is thought to recognize triacylated bacterial lipoproteins (as demonstrated by recognition of the synthetic molecule Pam3CSK4), gram positive bacterial peptidoglycans, and other microbiallyderived agonists shared with TLR1 (3, 6, 7). It is found as a homodimer or heterodimer with TLR1 or TLR2, associating through a region within the extracellular LRR repeats (2, 3, 7). It interacts directly with MYD88, leading to production of inflammatory cytokines (2, 7). Source: Recombinant protein corresponding to human TLR10 Fc Chimera, CHO-derived. Molecular Weight: ~115-135kD Endotoxin: <0.10EU/1ug (LAL method). Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human TLR10 Fc Chimera is immobilized at 1ug/mL (100uL/well), the concentration of Recombinant Human TLR1 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-3ug/mL. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
