TLR11, Fc Chimera, Recombinant, Mouse (Toll-like Receptor 11, Gm287)
Biozol Catalog Number:
USB-145845
Supplier Catalog Number:
145845
Alternative Catalog Number:
USB-145845-50
Manufacturer:
US Biological
Category:
Molekularbiologie
TLR11 is a type I transmembrane receptor of the Tolllike receptor family that is primarily expressed in epithelial cells in the liver, kidney, bladder and intestines, as well as in dendritic cells and macrophages (13). The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain (ECD) with 10 leucinerich repeats and 9 potential Nglycosylation sites, a 21aa transmembrane domain, and a 184aa cytoplasmic domain with a TIR domain (1). Within the ECD, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. Some researchers have found TLR11 in the dendritic cell plasma membrane, where it cooperates with MyD88 to take up antigen and initiate cell signaling (35). Others have found it in the endoplasmic reticulum (ER), where it interacts with the multispan ER protein UNC93B1 (6). TLR11 recognizes profilin proteins on Toxoplasma gondii and other intracellular parasites (3, 7). Binding of profiln activates dendritic cell subsets to expand, mature, produce IL12, and present antigenic profilin peptides to T cells (24). TLR11 is also reported to recognize pathogenic bacteria in the urinary tract (1). In the intestines, it is expressed on M cells, where its recognition of pathogenic salmonella blocks their entry into Peyers patches (8). TLR11 allows mice to be resistant to Salmonella typhi, the organism causing typhoid fever, while humans are sensitive (9). Source: Recombinant protein corresponding to mouse TLR11 Fc Chimera, CHO-derived. Molecular Weight: ~115-135kD Endotoxin: <0.01EU/1ug (LAL method) Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
