CD54, Recombinant, Human (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)

Product Overview

• Article Name: CD54, Recombinant, Human (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)
• Biozol Catalog Number: USB-149625
• Supplier Catalog Number: 149625
• Alternative Catalog Number: USB-149625-10,USB-149625-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505aa transmembrane glycoprotein containing a 29aa cytoplasmic domain, a 23aa transmembrane domain, and a 453aa extracellular domain. Recombinant human ICAM-1 is a 49.5kD glycoprotein comprising the extracellular domain (453aa residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of ~72-80kD by SDS-PAGE analysis under reducing conditions. Biological Activity: ICAM-1 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at 25ug/ml ICAM-1 concentration. AA Sequence: QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFVLPAT PPQLVSPRVL EVDTQGTVVC SLDGLFPVSE AQVHLALGDQ RLNPTVTYGN DSFSAKASVS VTAEDEGTQR LTCAVILGNQ SQETLQTVTI YSFPAPNVIL TKPEVSEGTE VTVKCEAHPR AKVTLNGVPA QPLGPRAQLL LKATPEDNGR SFSCSATLEV AGQLIHKNQT RELRVLYGPR LDERDCPGNW TWPENSQQTP MCQAWGNPLP ELKCLKDGTF PLPIGESVTV TRDLEGTYLC RARSTQGEVT RKVTVNVLSP RYE Crossreactivity: Bacteria Quality Control: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability:: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 49.5
• Purity: 98% by SDS-PAGE gel and HPLC analyses. Endotoxin: 0.1ng/ug
• Form: Supplied as a lyophilized powder in 10mM sodium phosphate. No preservative added. Reconstitute with sterile dH2O to 0.1-1mg/ml.