Interleukin-4, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF-1, Binetrakin, Lymphocyte Stimulatory Factor 1, Pitrakinra, IL4)

Product Overview

• Article Name: Interleukin-4, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF-1, Binetrakin, Lymphocyte Stimulatory Factor 1, Pitrakinra, IL4)
• Biozol Catalog Number: USB-149629
• Supplier Catalog Number: 149629
• Alternative Catalog Number: USB-149629-2,USB-149629-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9kD globular protein containing 129aa. Source: Recombinant protein corresponding to human IL-4, expressed in E.coli. Biological Activity: Determined by the dose-dependent stimulation of human TF-1 cells. The ED50 was found to be 0.2ng/ml. Specific Activity: 5x10e6units/mg Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: ~98% (SDS-PAGE and HPLC)
• Form: Supplied as a lyophilized powder.