Fibrinogen Degradation Product (FDP) BioAssay(TM) ELISA Kit (Rabbit)

Product Overview

• Article Name: Fibrinogen Degradation Product (FDP) BioAssay(TM) ELISA Kit (Rabbit)
• Biozol Catalog Number: USB-152563
• Supplier Catalog Number: 152563
• Alternative Catalog Number: USB-152563-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Fibrinogen Degradation Product (FDP) BioAssay(TM) ELISA Kit (Rabbit) is a sandwich enzyme immunoassay for in vitro quantitative measurement of FDP in rabbit plasma. Detection Range: 0.312-20ng/ml Sensitivity: 0.143ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Sample Matrix: Plasma Kit Components: *152563A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *152563B: Standard, 2x1 vial 152563C: Standard Diluent, 1x20ml *152563D: Detection Reagent A, 1x120ul *152563E: Detection Reagent B, 1x120ul 152563F: Assay Diluent A, 1x12ml 152563G: Assay Diluent B, 1x12ml 152563H: TMB Substrate, 1x9ml *152563J: Positive Control, 4.33ng/ml, 1x1 vial 152563K: Stop Solution, 1x6ml 152563L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (152563K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *152563A, *152563B, *152563D, *152563E and *152563J at -20C. After reconstitution of *152563J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 152563J: Positive Control: Intended Use: The control vial is from a rabbit plasma sample known to contain FDP. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contains rabbit FDP in rabbit plasma (lyophilized powder). Note: The rabbit source material used in this product is from healthy rabbit of SPF standard (specific pathogen-free animals). Expected Values: 4.33ng/ml Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.