Ribonuclease A (RNASE1) BioAssay(TM) ELISA Kit (Bovine)

Product Overview

• Article Name: Ribonuclease A (RNASE1) BioAssay(TM) ELISA Kit (Bovine)
• Biozol Catalog Number: USB-153064
• Supplier Catalog Number: 153064
• Alternative Catalog Number: USB-153064-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Ribonuclease A (RNASE1) BioAssay(TM) ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm. The concentration of Ribonuclease A (RNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. The Ribonuclease A (RNASE1) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNASE1 in bovine serum, plasma, tissue homogenates and other biological fluids. Detection Range: 3.12-200ng/ml Sensitivity: 1.31ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *153064A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *153064B: Standard, 2x1 vial 153064C: Standard Diluent, 1x20ml *153064D: Detection Reagent A (green), 1x120ul *153064E: Detection Reagent B (red), 1x120ul 153064F: Assay Diluent A, 1x12ml 153064G: Assay Diluent B, 1x12ml 153064H: TMB Substrate, 1x9ml *153064J: Positive Control, 21.4ng/ml, 1x1vial (Lyophilized) 153064K: Stop Solution, 1x6ml 153064L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (153064K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *153064A, *153064B, *153064D, *153064E and *153064J at -20C. After reconstitution of *153064J, store at 4C and use within 5 days Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 153064J: Positive Control: Intended Use: The Positive Control is from a bovine serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The positive control vial contains bovine RNASE1 in bovine serum (lyophilized powder). Note: The bovine based serum used in this product are from healthy bovine of SPF standard (specific pathogen-free animals). Expected Values: 21.4ng/ml Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.