Semaphorin 3C (SEMA3C) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Semaphorin 3C (SEMA3C) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-153100
• Supplier Catalog Number: 153100
• Alternative Catalog Number: USB-153100-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Semaphorin 3C (SEMA3C) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA3C in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 78-5000pg/ml Sensitivity: 30pg/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *153100A: Microtiter Strips, 1x96wells, Pre-coated, ready to use *153100B: Standard, 2x1vial 153100C: Standard Diluent, 1x20ml *153100D: Detection Reagent A (green), 1x120ul *153100E: Detection Reagent B (red), 1x120ul 153100F: Assay Diluent A, 1x12ml 153100G: Assay Diluent B, 1x12ml 153100H: TMB Substrate, 1x9ml *153100J: Positive Control, 2340pg/ml, 1x1vial (Lyophilized) 153100K: Stop Solution, 1x6ml 153100L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (153100K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *153100A, *153100B, *153100D, *153100E and *153100J at -20C. After reconstitution of *153100J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 153100J - Positive Control: Intended Use: The control vials are from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vials contain human SEMA3C in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis. Expected Value: 2340pg/ml Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.