Recombinant protein corresponding to aa24-249 with a N-Terminal His-Tag from mouse PR3 expressed in E. coli Accession No: Q61096 Amino Acid Sequence: MGHHHHHHSGSEF-AVQASKIVGG HEARPHSRPY VASLQLSRFP GSHFCGGTLI HPRFVLTAAH CLQDISWQLV TVVLGAHDLL SSEPEQQKFT ISQVFQNNYN PEENLNDVLL LQLNRTASLG KEVAVASLPQ QDQTLSQGTQ CLAMGWGRLG TQAPTPRVLQ ELNVTVVTFL CREHNVCTLV PRRAAGICFG DSGGPLICNG ILHGVDSFVI RECASLQFPD FFARVSMYVD WIQNVL Predicted Molecular Weight: 26.3kD Predicted Isoelectric Point: 6.5 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD. No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels, 7ul/well for large gels. Store at -20C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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