Source: Recombinant Human from E. coli Accession No: Q5GAN6 Fragment: Leu27~Gln216 (Accession No: Q5GAN6) Sequence: MGHHHHHHSGSEF-LHMA TAVLEESDQP LNEFWSSDSQ DKAEATEEGD GTQTTETLVL SNKEVVQPGW PEDPILGEDE VGGNKMLRAS ALFQSNKDYL RLDQTDRECN DMMAHKMKEP SQSCIAQYAF IHEDLNTVKA VCNSPVIACE LKGGKCHKSS RPFDLTLCEL SQPDQVTPNC NYLTSVIKKH IIITCNDMKR QLPTGQ Epitope Tag: N-terminal His-Tag Molecular Weight: 22.7kD Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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