Ribonuclease III, Nuclear (RNASEN) Recombinant, Human

Product Overview

• Article Name: Ribonuclease III, Nuclear (RNASEN) Recombinant, Human
• Biozol Catalog Number: USB-156664
• Supplier Catalog Number: 156664
• Alternative Catalog Number: USB-156664-10,USB-156664-50,USB-156664-200
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Recombinant protein corresponding to aa1114-1235 with an N-Terminal His-Tag from human RNASEN, expressed in E. coli (Q9NRR4) Amino Acid Sequence: MGHHHHHHSG S-IGVIFTH VRLLARAFTL RTVGFNHLTL GHNQRMEFLG DSIMQLVATE YLFIHFPDHH EGHLTLLRSS LVNNRTQAKV AEELGMQEYA ITNDKTKRPV ALRTKTLADL LESFIAALYI DKDLE Predicted Molecular Weight: 15.2kD Predicted Isoelectric Point: 6.9 Subcellular Location: Nucleus, nucleolus Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use, no need to heat, dilute or add reducing agents before use. Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier. Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel: Mini gels: 3-5ul/well Large gel: 7ul/well The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels. Store at -20C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 15.2
• UniProt: Q9NRR4
• Purity: 95%, Endotoxin: 1EU/ug
• Form: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.