Ribonuclease Inhibitor, Recombinant, Human (RI, RNase Inhibitor)
Biozol Catalog Number:
USB-156665
Supplier Catalog Number:
156665
Alternative Catalog Number:
USB-156665-10,USB-156665-50,USB-156665-200
Manufacturer:
US Biological
Category:
Molekularbiologie
Source: Recombinant Human from E. coli Accession No: P13489 Fragment: Asp218~Glu444 (Accession No: P13489) Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DLC GIVASKASLR ELALGSNKLG DVGMAELCPG LLHPSSRLRT LWIWECGITA KGCGDLCRVL RAKESLKELS LAGNELGDEG ARLLCETLLE PGCQLESLWV KSCSFTAACC SHFSSVLAQN RFLLELQISN NRLEDAGVRE LCQGLGQPGS VLRVLWLADC DVSDSSCSSL AATLLANHSL RELDLSNNCL GDAGILQLVE SVRQPGCLLE QLVLYDIYWS EEME Epitope Tag: N-terminal Tags: His-tag and T7-tag Molecular Weight: 28.4kD Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not v
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