Serum Amyloid P Component (SAP) Recombinant, Human
Biozol Catalog Number:
USB-156778
Supplier Catalog Number:
156778
Alternative Catalog Number:
USB-156778-10,USB-156778-50,USB-156778-200
Manufacturer:
US Biological
Category:
Molekularbiologie
Recombinant protein corresponding to aa20-223 with an N-Terminal His Tag from human PI16, expressed in E. coli. Swiss/UniProt Accession: P02743 Predicted Isoelectric Point: 6.4 Predicted Molecular Weight: 24.5kD Accurate Molecular Weight: 25kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Amino Acid Sequence: H TDLSGKVFVF PRESVTDHVN LITPLEKPLQ NFTLCFRAYS DLSRAYSLFS YNTQGRDNEL LVYKERVGEY SLYIGRHKVT SKVIEKFPAP VHICVSWESS SGIAEFWING TPLVKKGLRQ GYFVEAQPKI VLGQEQDSYG GKFDRSQSFV GEIGDLYMWD SVLPPENILS AYQGTPLPAN ILDWQALNYE IRGYVIIKPL VWV Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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