Sialic Acid Acetylesterase (SIAE) Recombinant, Human
Biozol Catalog Number:
USB-156786
Supplier Catalog Number:
156786
Alternative Catalog Number:
USB-156786-10,USB-156786-50,USB-156786-200
Manufacturer:
US Biological
Category:
Molekularbiologie
Recombinant protein corresponding to aa22~244 with an N-Terminal His-tag and GST-tag from Human SIAE expressed in E. coli. Swiss/UniProt Accession:Q9HAT2. Amino Acid Sequence: AGIGFRFAS YINNDMVLQK EPAGAVIWGF GTPGATVTVT LRQGQETIMK KVTSVKAHSD TWMVVLDPMK PGGPFEVMAQ QTLEKINFTL RVHDVLFGDV WLCSGQSNMQ MTVLQIFNAT RELSNTAAYQ SVRILSVSPI QAEQELEDLV AVDLQWSKPT SENLGHGYFK YMSAVCWLFG RHLYDTLQYP IGLIASSWGG TPIEAWSSGR SLKACGVPKQ GSIP Predicted Isoelectric Point: 6.5 Predicted Molecular Weight: 54.5kD Accurate Molecular Weight: 54kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted, Lyosome Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder in PBS, pH7.4, 5% trehalose, 0.01% sarcosyl. Reconstitute in sterile 10mM PBS, pH7.4 to a concentration of 0.1-1.0mg-ml. Do not vortex.
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