Fibroblast Growth Factor 18 (FGF18) BioAssay(TM) ECL ELISA Kit (Mouse)

Product Overview

• Article Name: Fibroblast Growth Factor 18 (FGF18) BioAssay(TM) ECL ELISA Kit (Mouse)
• Biozol Catalog Number: USB-157614
• Supplier Catalog Number: 157614
• Alternative Catalog Number: USB-157614-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Fibroblast Growth Factor 18 (FGF18) BioAssay(TM) ECL Kit is a sensitive chemiluminescent immunoassay for the in vitro quantitative measurement of FGF18 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 1.37-1000pg/ml Sensitivity: 0.61pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *157614A: Microtiter Plate, 1x96 wells, Pre-coated with antibody, ready to use. *157614B: Standard, 2x1vial 157614C: Standard Diluent, 1x20ml *157614D: Detection Reagent A, 1x120ul *157614E: Detection Reagent B, 1x120ul 157614F: Assay Diluent A, 1x12ml 157614G: Assay Diluent B, 1x12ml 157614H: Substrate A, 1x10ml *1576148J: Positive Control, 1x1vial, 28.3pg/ml (Lyophilized) 157614K: Substrate B, 1x2ml 157614L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *157614A, *157614B, *157614D, *157614E and *157614J at -20C. After reconstitution of *157614J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 157614J: Positive Control: Intended Use: The positive control is from mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contains mouse FGF18 in mouse serum (lyophilized powder). Note: The mouse based serum used in this product are from healthy mouse of SPF standard (Specific Pathogen-Free animals). Expected Values: 28.3pg/ml Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 100ul prepared Substrate Solution. Incubate 10 minutes at 37C. 8. Read RLU immediately.