Growth Differentiation Factor 11 (GDF11) (Human), BioAssay(TM) ECL ELISA Kit

Product Overview

• Article Name: Growth Differentiation Factor 11 (GDF11) (Human), BioAssay(TM) ECL ELISA Kit
• Biozol Catalog Number: USB-157680
• Supplier Catalog Number: 157680
• Alternative Catalog Number: USB-157680-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Growth Differentiation Factor 11 (GDF11) BioAssay(TM) ECL Kit is a sandwich Electrochemical luminescence-based immunoassay (ECL) suitable for use in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 1.37-1000pg/ml Sensitivity: 0.0.61pg/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *157680A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated with antibody, ready to use *157680B: Standard, 2x1vial 157680C: Standard Diluent, 1x10ml or 1x20ml *157680D: Detection Reagent A (green), 1x60ul or 1x120ul *157680E: Detection Reagent B (red), 1x60ul or 1x120ul 157680F: Assay Diluent A, 1x6ml or 1x12ml 157680G: Assay Diluent B, 1x6ml or 1x12ml 157680H: Substrate A, 1x5ml or 1x10ml *157680J: Positive Control, 36.5pg/ml, 1x1vial 157680K: Substrate B, 1x1ml or 1x2ml 157680L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution: The Stop Solution (157680K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *157680A, *157680B, *157680D, *157680E and *157680J at -20C. After reconstitution of *157680J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 157680J: Positive Control: Intended Use: The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: GDF11 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis. Expected Values: 36.5pg/ml Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 100ul prepared Substrate Solution. Incubate 10 minutes at 37C. 8. Read RLU immediately.