Biotin-11-dUTP, a biotinylated deoxynucleotide triphosphate analogue, can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart deoxythymidine triphosphate (dTTP). The resulting biotin-labeled DNA/cDNA probes are then detected with streptavidin-horseradish peroxidase (HRP) conjugates, alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Biotin-11-dUTP is commonly used for PCR, nick translation, as well as reverse transcription. It is also suitable for apoptosis assays. Synonyms: 2-Deoxy-5-[(1E)-3-[[6-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]-1-oxohexyl]amino]-1-propen-1-yl]uridine 5-(Tetrahydrogen triphosphate), Lithium (5-(4-amino-2-oxo-5-((E)-3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate CAS No: 1221498-88-9 Molecular Formula: C28H44Li4N7O16P3S Molecular Weight: 887.44 Appearance: Solution Purity: 90% Solubility: Water: 100mg/ml in H2O, EtOH: 46mg/ml, DMSO: 95 mg/ml Warm the tube at 37C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight:
887.44
Purity:
90%
Form:
Solution
CAS Number:
[1221498-88-9]
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