| AMIGO2 (amphoterininduced gene and ORF 2), also known as DEGA and Alivin1, is an approximately 65kD transmembrane cell adhesion protein. It belongs to a family of leucinerich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Mature human AMIGO2 consists of a 359aa extracellular domain (ECD) with six LRRs flanked by single LRRNT and LRRCT domains, followed by one immunoglobulinlike domain, a 21aa transmembrane segment, and a 103aa cytoplasmic domain (35). Within the ECD, human AMIGO2 shares 89% and 87% aa sequence identity with mouse and rat AMIGO2, respectively. AMIGO2 forms homodimers as well as heterodimers with the related AMIGO and AMIGO3 molecules (3, 6). Within the nervous system, AMIGO2 is transcribed in the cerebrum, hypothalamus, olfactory bulb, retina, hippocampus (pyramidal cells), and cerebellum (granule neurons and Purkinje cells) (3, 4, 7). It is also expressed in the liver, lung, testis, spleen, and small intestine (3, 4). AMIGO2 supports neuron survival, and is downregulated following proapoptotic stimulation (4). Its expression can be upregulated or downregulated in a variety of cancers, and antisense knockdown of AMIGO2 interferes with tumor cell adhesion to collagen as well as in vivo tumorigenicity (5). Source: Recombinant protein corresponding to aa39-393 from human, AMIGO2, CHO-derived (Accession Q86SJ2). Molecular Weight: ~90110kD Endotoxin: <0.10EU/1ug Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
