Transglutaminase 4, Recombinant, Human (Fibrinoligase, TGM4, TGP)
Biozol Catalog Number:
USB-167706
Supplier Catalog Number:
167706
Alternative Catalog Number:
USB-167706-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Transglutaminase 4, also known as TGM4, TG4, hTGP, or the prostatespecific transglutaminase, is a member of the transglutaminase family that is exclusively expressed and secreted from the prostate (1, 2). Transglutaminases catalyze the calciumdependent formation of isopeptide bonds between lysine and glutamine residues (3). In rodents, TGM4 is responsible for the formation of the copulatory plug and sperm antigenicity, but the function in humans is largely unknown (4). The expression of TGM4 is primarily regulated by retinoic acid and secondarily modulated by androgens (5). Recombinant protein corresponding to human Transglutaminase 4/TGM4, Met2-Lys684, with an N-terminal Met and 6-His tag, expressed in Spodoptera frugiperda, Sf 21 (baculovirus). Molecular Weight: ~78kD Biological Activity: Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine. The specific activity is >50 pmol/min/ug, as measured under the described conditions. N-terminal Sequence Analysis: No results obtained, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
