Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin, an attraction that is the strongest and most stable non-covalent interaction known. B-galactosidase (b-gal, b-gal) is an enzyme that hydrolyzes b-galactosides (such as lactose and various glycoproteins) into simple sugars. B-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. B-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified. Source: B-Galactosidase, expressed in E.coli. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:8000-1:32,000 Western Blot: 1:500-1:2500 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at 4C. Do Not Freeze Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified
Form:
Supplied as a liquid in 0.05M potassium phosphate, 0.001M magnesium chloride, 0.1M beta-mercaptoethanol, pH 7.8. Labeled with Biotin.
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