This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and FLISA. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000-1:5000 Flow Cytometry: 1:500-1:2500 FLISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with fluorescein isothiocyanate (FITC).
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