The pyruvate dehydrogenase complex contributes to transforming pyruvate into acetyl-CoA by a process called pyruvate decarboxylation. Recombinant protein corresponding to PDHE2, fused to His-Tag and GST-Tag, expressed in E. coli. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
48
Purity:
90%
Form:
Supplied as a liquid in 50mM Na2PO4, pH 7.5, 400mM NaCl, 1mM EDTA, 2mM DTT, 10% glycerol.
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