PD-L2 Inhibitor Screening BioAssay(TM) Kit (Biotin)

Product Overview

• Article Name: PD-L2 Inhibitor Screening BioAssay(TM) Kit (Biotin)
• Biozol Catalog Number: USB-171955
• Supplier Catalog Number: 171955
• Alternative Catalog Number: USB-171955-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-L2 Inhibitor Screening BioAssay(TM) Kit is designed for screening and profiling inhibitors of this signaling. This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L2, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader. Applications: This kit is most useful for screening for inhibitors of PD-L2. Components: PD-L1, Biotin-labeled, 1x5ug PD-L2, 1x100ug Streptavidin-HRP, 1x15ul 3x PD-1 Assay Buffer, 3x50ml Blocking Buffer, 1x50ml HRP chemiluminescent substrate A (transparent bottle), 1x6ml HRP chemiluminescent substrate B (brown bottle), 1x6ml White 96-well microplate, 1 plate Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.