PDE3A TR-FRET BioAssay(TM) Kit

Product Overview

• Article Name: PDE3A TR-FRET BioAssay(TM) Kit
• Biozol Catalog Number: USB-171964
• Supplier Catalog Number: 171964
• Alternative Catalog Number: USB-171964-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. The PDE3A TR-FRET BioAssay(TM) Kit comes in a convenient 96-well format, with purified PDE3A enzyme, fluorescently labeled PDE substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a microtiter plate are required for the PDE3A activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE3A for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then, fluorescence intensity can be measured using a fluorescence reader. The PDE3A TR-FRET Assay Kit is designed for identification of inhibitors of PDE3A using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader. Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications. Components: PDE3A recombinant enzyme, 1x1ug FAM-Cyclic-3, 5-AMP: 20um, 1x50ul PDE Assay Buffer, 1x25ml Tb donor, 1x30ml Binding Agent, 1x200ul Binding Buffer A, 1x20ml Binding Buffer B, 1x20ml Black, low binding NUNC microtiter plate, 1 plate Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.