Cell permeable, irreversible and non-toxic non-FMK pan-caspase inhibitor with improved potency, stability and toxicity over Z-VAD-FMK. Does not cross-react with cathepsins nor calpains. Non-toxic due to the 2,6- difluorophenoxy methyl (OPh) group. The peptide is not O-methylated to reduce hydrophobicity and to facilitate use in aqueous media. Inhibits ICE-family protease/caspase processing, leading to apoptosis and autophagy induction. Decreases proteasome activity. Used in apoptosis and inflammasome studies. Synonyms: Q-Val-Asp-OPh, pan-Caspase Inhibitor, N-(2-Quinolyl)-L-valyl-L-aspartyl-(2,6-difluorophenoxy) methylketone, (3S)-5-(2,6-Difluorophenoxy)-3-[[(2S)-3-methyl-1-oxo-2-[(2-quinolinylcarbonyl)amino]butyl]amino]-4-oxo-pentanoic Acid CAS No: 1135695-98-5 (anhydrous) Molecular Formula: C26H25F2N3O6 Sequence: Q-Val-Asp-OPh Molecular Weight: 513.5 Source: Synthetic Solubility: DMSO Biological Activity: The IC50 range for caspases -1, -3, -8, -9, -10, and -12 is 25-400nm. Purity: 95% (TLC) Appearance: White solid Note: Q-VD-OPh does not exhibit toxic effects at typical working concentrations: 10-20uM for in vitro cell culture applications, 10-120 (up to 1000) mg/kg for in vivo animal studies. Q-VD-OPh is a small hydrophobic compound and requires DMSO for solubilization. Stock solutions as high as 200mg/ml have been prepared in DMSO. To retain solubility in water at concentrations above 1mg/ml, 80 % DMSO is suggested. For tissue culture studies 10mM or 20mM stock solutions are prepared in DMSO and diluted 1:1000 directly into the tissue culture medium. For in vivo studies Q-VD-OPh has been administered in 80% to 100% DMSO to assure solubility at the doses given. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
513.5
Purity:
95% (TLC)
Form:
Supplied as a white solid.
CAS Number:
[1135695-98-5]
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