Hemagglutinin Influenza A Virus (Multiple strains) (H1N1 H1), Rabbit
Biozol Catalog Number:
USB-208947
Supplier Catalog Number:
208947
Alternative Catalog Number:
USB-208947-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA
Immunogen:
Recombinant protein corresponding to full-length hemeagglutinin H1 proteins, expressed in mammalian and avian viruses.
Influenza A (H1N1) virus is a subtype of influenza A virus and was the most common cause of human influenza (flu) in 2009. Some strains of H1N1 are endemic in humans and cause a small fraction of all influenza-like illness and a small fraction of all seasonal influenza. H1N1 strains caused a few percent of all human flu infections in 2004-2005. Other strains of H1N1 are endemic in pigs (swine influenza) and in birds (avian influenza). In June 2009, the World Health Organization declared the new strain of swine- origin H1N1 as a pandemic. This strain is often called swine flu by the public media. Swine influenza (also called swine flu, or pig flu) is an infection by any one of several types of swine influenza virus. Swine influenza virus (SIV) is any strain of the influenza family of viruses that is endemic in pigs. As of 2009, the known SIV strains include influenza C and the subtypes of influenza A known as H1N1, H1N2, H3N1, H3N2, and H2N3. The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin (H) and some 100 molecules of neuraminidase (N). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some non-structural protein molecules of uncertain function. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:5000 Western Blot: 1:500-1:2000 (ECL) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by Protein A affinity chromatography
Form:
Supplied as a lyophilized power from PBS, pH 7.4, 1% gelatin, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
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