Antibodies to small peptide/haptens are generally raised by coupling to a large carrier proteins such as KLH (Keyhole Limpet Hemocyanin). KLH mol wt ranges 3-7.5 million. It is used for carrier protein as it offers plenty of free amino, carboxyl and cysteines for coupling various peptides or haptens. Antibodies are produced to both KLH and the peptide/hapten. Anti-KLH may give non-specific signals in various immunoassays. Anti-KLH antibodies can be removed by solid phase immunoaffinity column chromatography over the KLH-Agarose affinity column or by adsorbing with KLH protein. Source: KLH, isolated from the hemolymphs of the molllusk Megathura crenulata Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a liquid in PBS with 11% glycerol.
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