Cholera Toxin, B Subunit, Vibrio cholerae (Choleragenoid) Not for Export, CAS [[131096-89-4]]

Product Overview

• Article Name: Cholera Toxin, B Subunit, Vibrio cholerae (Choleragenoid) Not for Export, CAS [[131096-89-4]]
• Biozol Catalog Number: USB-215930
• Supplier Catalog Number: 215930
• Alternative Catalog Number: USB-215930-500, USB-215930-1
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Produced by Vibrio cholerae, cholera toxin (CT) is a multimeric enterotoxin that transfers ADP-ribose to a G protein, locking adenyl cyclase in an on position. By binding to the membrane of enteric cells, cholera toxin stimulates the cellular adenylate cyclase system, causing the hyper-secretion of chloride and bicarbonate ions, which results in increased fluid secretion and the severe diarrhea characteristic of the disease cholera. The pentameric B subunit of CT binds with high efficiency to GM1 monosialoganglioside cell membrane receptors, present in many cell types, allowing its use experimentally in cell culture. Following binding, the toxin is endocytosed and travels retrogradely to the endoplasmic reticulum where the enzymatically active A subunit (CTA1) is translocated to the cytosol. Within the cytosol, CTA1 catalyzes the ADP-ribosylation of the Gs protein, activating adenylate cyclase and as a consequence increasing the intracellular concentration of cAMP. Relevant to the development of vaccine adjuvants, CT effectively modulates mammalian immune systems and acts as an adjuvant for co-administered antigens. Source: Cholera Toxin, B Subunit from Vibrio cholerae Molecular Weight: ~12kD Activity: Measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1ug of Cholera toxin B subunit/ml. Impurities: 0.5% Cholera toxin A subunit (SDS-PAGE) Solubility: H2O (10mg/ml) Protein: ~5% (Lowry) Storage and Stability: Lyophilized powder may be stored at 4C. Do not freeze. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Reconstituted product is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 12
• Purity: 95% (SDS-PAGE)
• Form: Supplied as a lyophilized powder in 0.05M Tris buffer, pH 7.5, 0.2M sodium chloride, 1mM sodium EDTA, 3mM sodium azide. Reconstitute with sterile ddH2O. Handle Gently. Do not vortex.
• CAS Number: [131096-89-4]