Beclin-1 Activator I, TAT-Beclin-1 (Atg6 Activator I, Beclin1-GAPR-1 Interaction Blocker I, Vps30 Activator I, H2N-YGRKKRRQRRR-GG-TNVFNATFEIWHDGEFGT-CO2H)

Product Overview

• Article Name: Beclin-1 Activator I, TAT-Beclin-1 (Atg6 Activator I, Beclin1-GAPR-1 Interaction Blocker I, Vps30 Activator I, H2N-YGRKKRRQRRR-GG-TNVFNATFEIWHDGEFGT-CO2H)
• Biozol Catalog Number: USB-217213
• Supplier Catalog Number: 217213
• Alternative Catalog Number: USB-217213-10
• Manufacturer: US Biological
• Category: Biochemikalien

Product Description

A cell-permeable autophagy-inducing peptide (Effective conc. 30 to 100uM) that is designed based on the essential HIV-1 virulence factor Nef-binding sequence of atg6/beclin1 and N-terminally fused to the HIV-1 Tat protein transduction domain sequence. Tat-Beclin1 is reported to compete against Beclin1 for binding to its negative regulator GAPR-1/GLIPR2 on the Golgi surface. Shown to effectively induce autophagy-mediated clearance of small (<1uM) CFP-htt103Q aggregates in HeLa cells (20uM/4h/d for 2 d) and inhibit RNA viral replications both in cultures (4h 10uM peptide treatment 4h post CHIKV, SINV, or WNV infection in HeLa cultures, 24h 0.5-5uM peptide pre-treatment before HIV infection in human MDM cultures) in vitro and in mice (15-20mg/kg/day i.p.) in vivo. The in vivo bioavailability of Tat-Beclin1 is limited to the peripheral tissues in mice, while a peptide based on Tat-Beclin1 retro-inverso sequence is demonstrated to effectively target CNS. Synonyms: Atg6 Activator I, Beclin1-GAPR-1 Interaction Blocker I, Vps30 Activator I, H2N-YGRKKRRQRRR-GG-TNVFNATFEIWHDGEFGT-CO2H, Autophagy Inducer IV Molecular Formula: C164H251N57O45 Sequence: H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg~Gly-Gly~Thr-Asn-Val-Phe-Asn-Ala-Thr-Phe-Glu-Ile-Trp-His-Asp-Gly-Glu-Phe-Gly-Thr-OH Solubility: DMSO or H2O Primary Target: GAPR-1/GLIPR2 Storage and Stability: Store at -20C under inert atmosphere. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Properties

• Purity: 97% (HPLC)
• Form: Supplied as a white powder.