NBD-Binding Peptide II, Cell Permeable (CTP-NBD, NEMO-Binding Domain Binding Peptide II, Cell-Permeable, CTP-NBD)

Product Overview

• Article Name: NBD-Binding Peptide II, Cell Permeable (CTP-NBD, NEMO-Binding Domain Binding Peptide II, Cell-Permeable, CTP-NBD)
• Biozol Catalog Number: USB-217579
• Supplier Catalog Number: 217579
• Alternative Catalog Number: USB-217579-5
• Manufacturer: US Biological
• Category: Biochemikalien

Product Description

A cell-permeable cytoplasmic transduction peptide (CTP, YGRRARRRARR) fused to the NEMO binding domain peptide (NBD, TALDWSWLQTE) that acts as a potent inhibitor of TNFa-stimulated NF-kB activity (IC50~50uM in HCT116 cells). Acts by interfering with the formation of active IKK complex and thereby prevents lkBa phosphorylation and its subsequent proteasomal degradation. Also blocks LPS-mediated increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activities. Colon-targeted capsules containing CTP-NBD are shown to reduce 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rodent models (4.5mg/kg/,p.o.) in vivo and diminish activities of myeloperoxidase and several inflammatory mediators. Formula: C123H197N47O32 Molecular Weight: 2846.2 Solubility: H2O (50mg/ml) Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 2846.2
• Purity: ~97% (HPLC)
• Form: Supplied as a white solid.